涓浗鑽悊瀛︿細鐮旂┒鍐冲畾锛屼簬2008骞10鏈堜腑鏃湪娌堥槼鍙紑鈥滀袱宀镐笁鍦拌嵂鐞嗗涓庝复搴婅嵂鐞嗗鏈細璁濄備細璁皢閭璇蜂袱宀镐笁鍦拌嵂鐞嗗棰嗗煙钁楀悕瀛﹁呭強鐩稿叧鐮旂┒浜哄憳鍙傚姞姝ゆ浼氳锛屽鍥藉唴澶栬嵂鐞嗗銆佷复搴婅嵂鐞嗗鍙戝睍鎬佸娍銆佺爺绌惰繘灞曟繁鍏ユ帰璁紝鏃ㄥ湪鍔犲己涓ゅ哺涓夊湴鑽悊瀛﹀悓琛岀殑瀛︽湳浜ゆ祦涓庡悎浣溿備細璁皢鐢变腑鍥借嵂鐞嗗浼氫笌杈藉畞鐪佽嵂瀛︿細鍏卞悓涓惧姙锛岀敱娌堥槼鑽澶у涓庝腑鍥藉尰绉戝ぇ瀛︽壙鍔炪
1. 浼氳鏃堕棿锛2008骞10鏈10鏃10鏈13鏃
2. 浼氳鍦扮偣锛 娌堥槼涓夐殕涓ぉ閰掑簵
3. 浼氳璇█锛氫腑鏂/鑻辨枃
4. 浼氳娉ㄥ唽锛800鍏冧汉姘戝竵/浜猴紙鐮旂┒鐢熷嚟瀛︾敓璇佸噺鍗婏級
5. 寰佹枃鍐呭锛氱缁忕簿绁炶嵂鐞嗐佽偪鐦よ嵂鐞嗐佸績鑴戣绠¤嵂鐞嗐佽嵂鐗╀唬璋㈠姩鍔涘銆佷复搴婅嵂鐞嗐佹姉鐐庡厤鐤嵂鐞嗐佹姉鐢熺礌鑽悊绛夌浉鍏崇爺绌跺唴瀹广
6. 寰佹枃瑕佹眰锛
锛1锛 鎵鏈夊綍鐢ㄧǹ浠跺皢鍒婄櫥鍦ㄨ嫳鏂囨湡鍒夾sian Journal of Pharmaceutical Sciences锛堛婁簹娲茶嵂鐗╁埗鍓傜瀛︺嬶級涓婏紱
锛2锛 鎶曠ǹ鎽樿鎴栧叏鏂囦弗鏍奸伒鐓ц鏉傚織鐨勬牸寮忚姹傦細鍖呮嫭鐮旂┒璁烘枃銆佷笓棰樻姤鍛娿傛憳瑕佷负鑻辨枃锛屾寜浠ヤ笅瑕佹眰鎾板啓锛屽嵆鍖呮嫭鈥滈鐩佺洰鐨勩佹柟娉曘佺粨鏋溿佺粨璁衡濈粍鎴愶紝瀛楁暟涓嶈秴杩500瀛椼傞噰鐢╓ord 鏍煎紡锛屽崟鍊嶈璺濓紝灏忓洓鍙峰瓧锛孉4绾搞傚瓧浣撲负Times New Roman銆傚彲鍙傜収闄勪欢鏍煎紡杩涜鍑嗗銆傦紙璇﹁鍚庨檮鎽樿鑼冧緥锛屽叏鏂囪寖渚嬭鐧婚檰http://www.asianjps.com/en/qkjs.asp锛
7. 浼氳鏈熼棿灏嗙粍缁囦紭绉闈掑勾鑽悊瀛﹀伐浣滆呬紭绉璁烘枃璇勫娲诲姩锛岃鍦ㄥ洖鎵т腑鏍囨槑鏄惁鍙傚姞姝よ瘎濂栨椿鍔ㄣ
8. 璁烘枃寰佺ǹ鎴鏃ユ湡涓2008骞9鏈15鏃ワ紝璇峰皢璁烘枃鎽樿鎴栧叏鏂嘐mail鑷筹細鐜嬭姵zhongyaoyaoli@gmail.com銆
9. 浼氳鎶ュ埌锛
锛1锛 鏃 闂达細2008骞10鏈10鏃ュ叏澶
锛2锛 鍦 鐐癸細娌堥槼涓夐殕涓ぉ閰掑簵 娌堥槼甯傚拰骞冲尯鍖楀ぇ琛91鍙 鐢佃瘽锛024-22701111
锛3锛 涔樿溅璺嚎锛
娌堥槼鍖楃珯锛氫箻鍧203璺叕浜よ溅鍒颁竷绾矾绔欎笅杞﹀嵆鍒帮紱鎴栦箻鍧愬嚭绉熸苯杞︼紝杞﹁垂10鍏冨乏鍙炽
娌堥槼妗冧粰鏈哄満锛氫箻鍧愭満鍦哄反澹埌缁堢偣绔欙紙椹矾婀剧珯锛変笅杞︼紝姝ヨ8-10 鍒嗛挓鍗冲埌浼氬満鈥斺旀矆闃充笁闅嗕腑澶╅厭搴椼
10. 椋熷瀹夋帓
椋熷鐢变細鍔＄粍缁熶竴瀹夋帓锛岃垂鐢ㄨ嚜鐞嗭紝鐢辨墍鍦ㄥ崟浣嶆寜瑙勫畾鎶ラ攢銆備綇瀹挎爣鍑嗭細鍑嗗洓鏄熺骇锛屽弻浜烘爣鍑嗛棿锛堝崟搴/鍙屽簥锛夛紱浼氳浼樻儬浠锋牸锛248鍏/澶╋紱涓汉鍏ヤ綇浠锋牸锛368鍏/澶╋紱鐢ㄩ鍦扮偣, 鐢变細鍔＄粍缁熶竴瀹夋帓銆
11. 浼氳鍥炴墽
璇锋偍鍦ㄧ櫨蹇欎腑濉啓涓嬭〃锛屽苟璇蜂簬9鏈10鏃ヤ箣鍓嶄紶鐪熸垨鐢佃瘽銆丒-mail涓庡瓩鏄庣珛鑱旂郴锛堣瑙佷細璁仈绯绘柟寮忥級锛屼互渚夸細璁畨鎺掋
鍙 浼 鍥 鎵
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濮撳悕
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鎬у埆
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姘戞棌
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骞撮緞
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宸ヤ綔鍗曚綅
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鑱岀О/鑱屽姟
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鑱旂郴鍦板潃
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閭紪
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鐢佃瘽
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鎵嬫満
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鐢靛瓙閭
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鏄惁鍙傚姞浼樼璁烘枃璇勯
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鏄
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鍚
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鎴块棿绫诲埆锛堝潎涓248鍏/澶╋紱璇烽夋嫨浣忓绫诲埆锛涘鏈夊悎浣忎汉锛岃娉ㄦ槑锛夛細
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鍟嗗姟鏍囬棿锛堝弻搴婏級
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鏄惁鍚堜綇
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鍚堜綇浜
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鍟嗗姟鏍囬棿锛堝崟搴婏級
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澶囨敞
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12. 浼氳鑱旂郴鏂瑰紡
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鑱旂郴浜
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鐜 鑺
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瀛欐槑绔
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鍦 鍧
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娌堥槼甯傛矆娌冲尯鏂囧寲璺103鍙 娌堥槼鑽澶у 31# 鑽悊鏁欑爺瀹
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杈藉畞鐪佹矆闃冲競鍜屽钩鍖哄寳浜岄┈璺92鍙 涓浗鍖荤澶у鑽悊鏁欑爺瀹
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閭 缂
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110016
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110001
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鐢 璇
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024-82256261
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13358866401锛024-23256666-5317
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浼 鐪
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024-23986339
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024-24254188
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Email
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zhongyaoyaoli@gmail.com
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sunmingli1980817@yahoo.com.cn
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澶囨敞
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浼氳鎶曠ǹ璇峰彂鑷虫淇＄
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浼氳鍥炴墽璇峰彂鑷虫淇＄
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13. 浼氳鎽樿鑼冧緥
Study of the expression of EGFP-TK gene loaded PLGA-nanoparticles in hepatocarcinoma cells
Ji Liu1, Zhirong Zhang2, Zhirong Zhong23, Qin He2*
1School of the Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China
2West China School of Pharmacy, Sichuan University, Chengdu 610041, China
3Lu Zhou Medical College, Luzhou 646000, China
Abstract
Purpose: To prepare EGFP-TK (enhanced green fluorescent protein-thymidine kinase) gene nanoparticles and investigate the expression in hepatocarcinoma cells. Methods: Biodegradable and biocompatible polymer PLGA was used to prepare nanoparticles by the double-emulsion evaporation technique and the characteristics of the nanoparticles were investigated, including morphology, and entrapment efficiency. The expression of EGFP in human hepatocarcinoma SMMC-7221 cells and human normal parenchymal Chang liver cells was assessed by flow cytometric analysis and confocal laser scanning microscopy. The expression of TK gene was investigated by the Trypan blue cell counting method which was able to determine the cells dying after the addition of the drug gancyclovir (GCV). Results: The resulting nanoparticles had a regular spherical surface and a narrow particle size with a mean diameter of 72 nm. The average entrapment efficiency was 91.25%. The EGFP and TK gene expression of DNA-PLGA-nanoparticles was much higher than that of naked DNA. It was able to protect plasmid while prolonging its release. Conclusions: The enhanced transfection efficiency and greater ability to prolong the release of plasmid DNA are due to the nanoparticle encapsulation.
Keywords: EGFP-gene Preparation Nanoparticles Expression
涓浗鑽悊瀛︿細/杈藉畞鐪佽嵂瀛︿細
浜孫O鍏勾鍏湀鍗佹棩
浜叕缃戝畨澶 11010202007922鍙
鎵撳嵃